Ecl Western Blot Detection System

Ecl western blotting system uses horseradish peroxidase hrp conjugated anti mouse and anti rabbit antibodies for luminol based detection of western blots.

Ecl western blot detection system.

Blots probed with mouse or rabbit primary antibodies are incubated with hrp conjugated. Improving the overall efficiency of electrophoresis systems these reagents. Pierce ecl western blotting substrate provides reliability and performance equivalent to other standard ecl substrates for detection of hrp enzyme activity. Amersham ecl western blotting system is designed for detection of mouse and rabbit primary antibodies on western blots using hrp conjugated secondary antibodies and ecl reagentsnucleic acids or proteins in a complex sample can be visualized using electrophoresis and blotting procedures and the compatible reagents.

Contents include the following. It enables the detection of picogram amounts of antigen and allows for easy detection of hrp using photographic or other imaging methods. Ecl substrates for high sensitivity western blot detection chemiluminescent assays depend on the emission of light as a product of a chemical reaction and are commonly used for the detection of proteins on western blots. Expression and purification of gst tagged proteins can be monitored by western blot analysis using amersham ecl amersham ecl prime or amersham ecl select detection systems to enhance sensitivity.

Each system contains the following reagents sufficient for detection of 1000 cm membrane. General description ecl western blotting system is the world s most widely used and referenced chemiluminescent immunodetection system. Ecl western blotting detection reagents in combination with hybond ecl membranes form a complete solution for western blot applications. Anti mouse hrp conjugate 100 µl anti rabbit hrp conjugate 100 µl ecl detection reagents 1 and 2 62 5 ml each blocking agent 5 g.